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Ex vivo oculomotor skive kultur fra embryonale GFP-udtrykker mus for tidsforskudt billeddannelse af oculomotor nerve outgrowth
 

Ex vivo oculomotor skive kultur fra embryonale GFP-udtrykker mus for tidsforskudt billeddannelse af oculomotor nerve outgrowth

Article doi: 10.3791/59911
July 16th, 2019

Summary July 16th, 2019

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En ex vivo skive analyse tillader oculomotoriske nerve udvækst at blive afbildet i realtid. Udsnit genereres ved at indlejre E 10.5 ISLMN: gfp -embryoner i agopstået, udskæring på en vibratome og vokser i en scene-top inkubator. Axon-vejlednings forløbene vurderes ved at tilføje inhibitorer til kultur medierne.

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