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Optimalisering, design og unngå fallgruver i manuell multiplex fluorescerende immunhistokjemi
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Optimalisering, design og unngå fallgruver i manuell multiplex fluorescerende immunhistokjemi

Article DOI: 10.3791/59915-v • 09:15 min • July 26th, 2019
July 26th, 2019 •
1, 1, 1, 1, 1, 2, 3, 2, 1, 1, 1
1Department of Surgery, University of Michigan, 2Department of Molecular and Cellular Physiology, University of Michigan, 3Department of Pathology, University of Michigan

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Multiplex fluorescerende immunhistokjemi er en ny teknologi som muliggjør visualisering av flere celletyper innenfor intakt formalin, parafin embedded (FFPE) vev. Presentert er retningslinjer for å sikre en vellykket 7-farge multiplex med instruksjoner for å optimalisere antistoffer og reagenser, forberede lysbilder, design og tips for å unngå vanlige problemer.

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Kreftforskning kreft metastasering immunofluorescence multiplex fluorescerende immunhistokjemi mikromiljøet celle-til-celle-engasjement farging fluoroforen
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