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Otimização, design e evitando armadilhas em manual multiplex fluorescente imuno-histoquímica
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Otimização, design e evitando armadilhas em manual multiplex fluorescente imuno-histoquímica

Article DOI: 10.3791/59915-v • 09:15 min • July 26th, 2019
July 26th, 2019 •
1, 1, 1, 1, 1, 2, 3, 2, 1, 1, 1
1Department of Surgery, University of Michigan, 2Department of Molecular and Cellular Physiology, University of Michigan, 3Department of Pathology, University of Michigan

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Multiplex fluorescente imunoistoquímica é uma tecnologia emergente que permite a visualização de vários tipos de células dentro de formalina intacta-fixo, parafina incorporado (FFPE) tecido. Apresentados são diretrizes para garantir um multiplex de 7 cores bem sucedido com instruções para otimizar anticorpos e reagentes, preparando slides, design e dicas para evitar problemas comuns.

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Pesquisa do cancro edição 149 cancro metástase imunofluorescência immunohistochemistry fluorescente multiplex microambiente acoplamento Cell-to-Cell mancha fluoróforo
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