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JoVE Journal Cancer Research
Optimering, design och undvika fallgropar i manuella multiplex fluorescerande immunohistokemi
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Optimering, design och undvika fallgropar i manuella multiplex fluorescerande immunohistokemi

Article DOI: 10.3791/59915-v • 09:15 min • July 26th, 2019
July 26th, 2019 •
1, 1, 1, 1, 1, 2, 3, 2, 1, 1, 1
1Department of Surgery, University of Michigan, 2Department of Molecular and Cellular Physiology, University of Michigan, 3Department of Pathology, University of Michigan

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Multiplex fluorescerande immunohistokemi är en framväxande teknik som möjliggör visualisering av flera celltyper inom intakt formalin-fast, paraffin inbäddade (FFPE) vävnad. Presenteras är riktlinjer för att säkerställa en lyckad 7-Color multiplex med instruktioner för att optimera antikroppar och reagenser, förbereda diabilder, design och tips för att undvika vanliga problem.

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Cancerforskning fråga 149 cancer metastaser immunofluorescens multiplex fluorescerande immunohistokemi microenvironment cell-till-cell engagemang färgning fluorophore
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