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Journal / Biochemistry / Måling af interaktioner mellem fluorescerende…
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Biochemistry

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JoVE Journal Biochemistry
Måling af interaktioner mellem fluorescerende sonder og lignin i plante sektioner af sFLIM baseret på naturlig Auto Luorescens
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Måling af interaktioner mellem fluorescerende sonder og lignin i plante sektioner af sFLIM baseret på naturlig Auto Luorescens

Article DOI: 10.3791/59925-v • 07:15 min • January 2nd, 2020
January 2nd, 2020 •
1, 2, 2, 3
1PICT Platform, Université de Reims Champagne-Ardenne, 2Fractionation of AgroResources and Environment (FARE) Laboratory, INRA, Université de Reims Champagne-Ardenne, 3Unité de Glycobiologie Structurale et Fonctionnelle, Université de Lille

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Denne protokol beskriver en original opsætning, der kombinerer spektral-og fluorescens livstids målinger for at evaluere Förster resonans energioverførsel (FRET) mellem de rhodamine baserede fluorescerende sonder og lignin polymer direkte i tykke plante sektioner.

Tags

Biokemi sFLIM fluorescens levetid FRET Autofluorescens lignocellulose interaktion
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