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Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate
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Measuring Relative Insulin Secretion using a Co-Secreted Luciferase Surrogate

Article DOI: 10.3791/59926-v • 05:58 min • June 25th, 2019
June 25th, 2019 •
1, 1
1Department of Pharmacology, University of Texas Southwestern Medical Center

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This protocol describes how to perform rapid low-cost luciferase assays at medium-throughput using an insulin-linked Gaussia luciferase as a proxy for insulin secretion from beta cells. The assay can be performed with most luminescence plate readers and multichannel pipettes.

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Measuring Relative Insulin Secretion Co-secreted Luciferase Surrogate Assay Beta Cell Lines Drug Discovery Characterization Multiple Treatments Immediate Results Affordable Small Molecule Modulators Diabetes Therapies Beta Cell Function Protein Secretion Neurotransmitter Secretion Lentiviral System Human Islets Cell Culture Multichannel Pipettes Glucose Response Insulin ELISA Krebs-Ringer Bicarbonate Buffer Gaussia Luciferase Working Solution MIN6 Cells Plating
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