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JoVE Journal Chemistry
NMR-based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases
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NMR-based Activity Assays for Determining Compound Inhibition, IC50 Values, Artifactual Activity, and Whole-Cell Activity of Nucleoside Ribohydrolases

Article DOI: 10.3791/59928-v • 10:24 min • June 30th, 2019
June 30th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,2, 1,3
1Department of Chemistry, Adelphi University, 2Department of Chemistry, Washington University in St. Louis, 3Department of Chemistry, Boston University

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NMR-basierte Aktivitätsassays wurden entwickelt, um Inhibitoren von zwei Nukleosid-Ribohydrolase-Enzymen zu identifizieren und zu charakterisieren. Es werden Protokolle für anfängliche zusammengesetzte Assays mit 500 m und 250 M, Dosis-Wirkungs-Assays zur Bestimmung der IC-50-Werte, Waschmittel-Gegensieb-Assays, Jump-Dilution-Zähler-Screen-Assays und Assays in E. coli-Vollzellen bereitgestellt.

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Chemie Ausgabe 148 Waschmittelzähler Enzymaktivitätstest IC 50-Wert Sprungverdünnung 1H NMR 19F NMR Ganze Zelle NMR Nukleosidribohydrolase
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