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Journal / Genetics / High-density DNA en RNA micro…
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Genetics

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JoVE Journal Genetics
High-density DNA en RNA micro arrays-Photolithografische synthese, hybridisatie en voorbereiding van grote nucleïnezuur bibliotheken
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High-density DNA en RNA micro arrays-Photolithografische synthese, hybridisatie en voorbereiding van grote nucleïnezuur bibliotheken

Article DOI: 10.3791/59936-v • 11:22 min • August 12th, 2019
August 12th, 2019 •
1, 1, 1, 1, 1
1Institute of Inorganic Chemistry, Faculty of Chemistry, University of Vienna

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In dit artikel presenteren en bespreken we nieuwe ontwikkelingen in de synthese en toepassingen van nucleïnezuur micro arrays die in situ zijn gefabriceerd. Concreet laten we zien hoe de protocollen voor DNA-synthese kunnen worden uitgebreid naar RNA en hoe micro arrays kunnen worden gebruikt om opvraagbaar nucleïnezuur bibliotheken te creëren.

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Genetica probleem 150 Microarray foto lithografie in situ synthese fosforamidtische chemie acetale levulinyl Ester fluorescentie splijtbaar substraten DNA-gegevensopslag digitale informatie
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