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Genetics

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JoVE Journal Genetics
High-density DNA og RNA-mikroarrays-Photolithographic syntese, hybridisering og fremstilling af store nukleinsyre biblioteker
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High-density DNA og RNA-mikroarrays-Photolithographic syntese, hybridisering og fremstilling af store nukleinsyre biblioteker

Article DOI: 10.3791/59936-v • 11:22 min • August 12th, 2019
August 12th, 2019 •
1, 1, 1, 1, 1
1Institute of Inorganic Chemistry, Faculty of Chemistry, University of Vienna

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I denne artikel præsenterer og diskuterer vi nye udviklinger i syntesen og anvendelser af nukleinsyre-mikroarrays, som er fabrikeret in situ. Konkret viser vi, hvordan protokollerne for DNA-syntese kan udvides til RNA, og hvordan mikromatricer kan bruges til at oprette hentes nukleinsyre-biblioteker.

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Genetik microarray photolithography in situ syntese phosphoramidite kemi acetalplast levulinyl ester fluorescens kløvende substrater DNA datalagring digital information
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