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Journal / Biochemistry / Extremely Rapid and Specific Metabolic Labelling…
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Biochemistry

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JoVE Journal Biochemistry
Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)
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Extremely Rapid and Specific Metabolic Labelling of RNA In Vivo with 4-Thiouracil (Ers4tU)

Article DOI: 10.3791/59952-v • 11:46 min • August 22nd, 2019
August 22nd, 2019 •
1, 1
1Wellcome Centre for Cell Biology, School of Biological Sciences, University of Edinburgh

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Summary
Automatic Translation

The use of thiolated uracil to sensitively and specifically purify newly transcribed RNA from the yeast Saccharomyces cerevisiae.

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Metabolic Labelling RNA 4-Thiouracil Ers4tU Pulse-chase Experiments Protein Depletion Best AID 4U Protocol Background Labeling Times Yeast Growth Methanol Sample Volume Zirconia Beads S.pombe Spike Thiolated Cells Concentration Time Course
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