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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
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Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Article DOI: 10.3791/59955-v • 11:30 min • March 24th, 2020
March 24th, 2020 •
*1, 1, 1, 2
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program, National Institute of Environmental Health Sciences
* These authors contributed equally

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Summary
Automatic Translation

This protocol describes several procedures for preparing high quality frozen tissue samples at the time of necropsy for use in the comet assay to assess DNA damage: 1) minced tissue, 2) scraped epithelial cells from the gastrointestinal tract, and 3) cubed tissue samples, requiring homogenization using a tissue mincing device.

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Frozen Tissue Comet Assay DNA Damage Evaluation Logistics Remote Laboratory Tissue Analysis Personnel Training Technical Errors Mechanical DNA Damage Pre-clinical Drug Development Safety Assessment Environmental Bio-monitoring Genotoxic Potential DNA Repair Protective Effects Chemotherapy Radiation Food Additives
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