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Journal / Developmental Biology / Genoyping og kvantificering af In Situ…
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Developmental Biology

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JoVE Journal Developmental Biology
Genoyping og kvantificering af In Situ Hybridization Farvning i Zebrafisk
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Genoyping og kvantificering af In Situ Hybridization Farvning i Zebrafisk

Article DOI: 10.3791/59956-v • 05:41 min • January 28th, 2020
January 28th, 2020 •
*1,2, *1,2, 1,2,3
1MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, 2BHF Centre of Research Excellence, 3Institute of Cancer and Genomic Sciences, University of Birmingham
* These authors contributed equally

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Genredigeringsteknologier har gjort det muligt for forskere at generere zebrafiskmutanter til at undersøge genfunktionen med relativ lethed. Her giver vi en guide til at udføre parallelle embryo genotyping og kvantificering af in situ hybridisering signaler i zebrafisk. Denne upartiske tilgang giver større nøjagtighed i fænotypiske analyser baseret på in situ hybridisering.

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Udviklingsmæssige Biologi genoyping in situ hybridisering zebrafisk mutanter bias billedkvantificering
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