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Journal / Genetics / Identifiera mutationer genom högupplöst smältning …
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JoVE Journal Genetics
Identifiera mutationer genom högupplöst smältning i en TILLING population av ris
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Identifiera mutationer genom högupplöst smältning i en TILLING population av ris

Article DOI: 10.3791/59960-v • 06:10 min • September 2nd, 2019
September 2nd, 2019 •
1, 1, 1, 2, 3, 1, 4
1National Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, 2Jiaxing Academy of Agricultural Sciences, 3Institute of Nuclear Agricultural Sciences, Zhejiang University, 4The New Countryside Development Institute at Zhejiang University

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I denna artikel presenterar vi det protokoll som beskrivs som högupplöst smält analys (HRM)-baserade mål inducerade lokala lesioner i genom (TILLING). Denna metod utnyttjar fluorescensförändringar under smältningen av DNA duplex och är lämplig för hög dataflöde screening av både införande/radering (indel) och Single Base substition (SBS).

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Genetik högupplöst smältning analys HRM TILLING mutation screening indel och SBS gammastrålar ris
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