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Introduktion af et gen knockout direkte i den Amastigote fase af Trypanosoma cruzi ved hjælp af Crispr/Cas9 system
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Introduktion af et gen knockout direkte i den Amastigote fase af Trypanosoma cruzi ved hjælp af Crispr/Cas9 system

Article DOI: 10.3791/59962-v • 06:13 min • July 31st, 2019
July 31st, 2019 •
1, 1, 1, 1
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

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Her beskriver vi en protokol til at introducere en gen knockout i den ekstracellulære amastigote af Trypanosoma cruzi, ved hjælp af crispr/Cas9 system. Væksten fænotype kan følges op enten ved celletælling af axeniske amastigote kultur eller ved spredning af intracellulære amastigotes efter vært celle invasion.

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Genetik crispr/Cas9 gen knockout Stage-specifikke Trypanosoma cruzi Chagas sygdom kinetoplastid parasit Drug Target axeniske kultur amastigote
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