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Journal / Developmental Biology / Serum fri produktion af tredimensionale humane…
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Developmental Biology

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JoVE Journal Developmental Biology
Serum fri produktion af tredimensionale humane Hepatokugler fra pluripotente stamceller
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Serum fri produktion af tredimensionale humane Hepatokugler fra pluripotente stamceller

Article DOI: 10.3791/59965-v • 06:57 min • July 20th, 2019
July 20th, 2019 •
1, 1,2, 1,3, 1,4, 1, 1, 4, 3, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health, University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin

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Denne protokol beskriver en tilgang til fremstilling af hepatokugler fra humane pluripotente stamceller ved hjælp af et defineret kultur system og celle selvsamling. Denne protokol er reproducerbar i en række cellelinjer, omkostningseffektiv og giver mulighed for produktion af stabile humane hepatospheres til biomedicinsk anvendelse.

Tags

Udviklingsmæssige biologi pluripotente stamceller tredimensionel kultur hepatokugler Drug metabolisme og protein sekretion stabil celle fænotype defineret kultur system
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