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Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry
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Cell Cycle-specific Measurement of γH2AX and Apoptosis After Genotoxic Stress by Flow Cytometry

Article DOI: 10.3791/59968-v • 08:21 min • September 1st, 2019
September 1st, 2019 •
1,2, 1,2, 1,2, 1,2, 1,2,3, 1,2
1CCU Molecular and Radiation Oncology, German Cancer Research Center, 2Department of Radiation Oncology, Heidelberg University Hospital, 3Department of Radiation Oncology, Freiburg University Medical Center

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Summary
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The presented method combines the quantitative analysis of DNA double-strand breaks (DSBs), cell cycle distribution and apoptosis to enable cell cycle-specific evaluation of DSB induction and repair as well as the consequences of repair failure.

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Cell Cycle-specific &947;H2AX Apoptosis Genotoxic Stress Flow Cytometry Treatment Effects Cellular Proliferation Coping Mechanisms DNA Damage Response Double-strand Break Recognition And Repair Cell Death Comprehensive Assay Radiochemotherapeutic Treatments Clinical Oncology Radiobiology Radiotherapy Correlative Studies Culture Cells Fixation Solution Single Cell Suspension Adherence Cells 70% Ethanol
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