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Journal / Cancer Research / 细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定…
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JoVE Journal Cancer Research
细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定
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细胞周期特异性测量βH2AX和细胞凋亡后热源毒性应力通过流细胞测定

Article DOI: 10.3791/59968-v • 08:21 min • September 1st, 2019
September 1st, 2019 •
1,2, 1,2, 1,2, 1,2, 1,2,3, 1,2
1CCU Molecular and Radiation Oncology, German Cancer Research Center, 2Department of Radiation Oncology, Heidelberg University Hospital, 3Department of Radiation Oncology, Freiburg University Medical Center

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该方法结合了DNA双链断裂（DSB）、细胞循环分布和凋亡的定量分析，实现了对DSB诱导和修复以及修复失败后果的细胞周期特定评估。

Tags

癌症研究，第151期，DNA双链断裂，细胞周期分布，凋亡，DNA损伤反应，电离辐射，碳电离辐射，基因毒性应激，流式细胞测定
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