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Journal / Genetics / Omdanne, genomredigering og Phenotyping…
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Genetics

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JoVE Journal Genetics
Omdanne, genomredigering og Phenotyping kvælstofbindende tropisk Cannabaceae træ Parasponia andersonii
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Omdanne, genomredigering og Phenotyping kvælstofbindende tropisk Cannabaceae træ Parasponia andersonii

Article DOI: 10.3791/59971-v • 12:22 min • August 18th, 2019
August 18th, 2019 •
1,2, 1,2, 1,3, 1,4, 1, 1, 1, 1
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 2Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

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Parasponia andersonii er et hurtigtvoksende tropisk træ, der tilhører cannabis familien (Cannabaceae) og kan danne kvælstofbindende rodknuder i forbindelse med Rhizobium. Her beskriver vi en detaljeret protokol for omvendte genetiske analyser i P. andersonii baseret på Agrobacterium tumefaciens-mediated stabil transformation og crispr/Cas9-baseret genomredigering.

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Genetik Parasponia andersonii cannabaceae træ træ Agrobacterium tumefaciens stabil transformation crispr-Cas9 genomredigering nodulation Rhizobium mykorrhization
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