Chapters
Summary
Parasponia andersonii is a fast-growing tropical tree that belongs to the Cannabis family (Cannabaceae) and can form nitrogen-fixing root nodules in association with the rhizobium. Here, we describe a detailed protocol for reverse genetic analyses in P. andersonii based on Agrobacterium tumefaciens-mediated stable transformation and CRISPR/Cas9-based genome editing.
Tags
Transforming Genome Editing Phenotyping Nitrogen-fixing Tropical Tree Parasponia Andersonii Cannabis Family Rhizobium Symbiosis Comparative Studies Genetic Networks Stable Transgenic Mutant Lines In Vitro Propagation Tissue Culture Molecular Cloning Research Model Germinating Seeds Disinfect Seeds Hypochlorite Bleach Sterilized Water PCR Tubes
PLAYLIST
CRISPR- genomic scissors of the modern era
Tweaking the genome using the CRISPR/Cas9 suite
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Research • BiologyGeneration of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9 -
Research • GeneticsCRISPR-mediated Genome Editing of the Human Fungal Pathogen Candida albicans -
Research • GeneticsCell Surface Receptor Identification Using Genome-Scale CRISPR/Cas9 Genetic Screens -
Research • BioengineeringCRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art -
Research • GeneticsCRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
CRISPR in Cancer
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Research • Cancer ResearchUsing CRISPR/Cas9 to Knock Out GM-CSF in CAR-T Cells -
Research • Cancer ResearchVirus Delivery of CRISPR Guides to the Murine Prostate for Gene Alteration -
Research • Cancer ResearchInvestigation of Genetic Dependencies Using CRISPR-Cas9-based Competition Assays -
Research • Cancer ResearchUsing CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells -
Research • GeneticsInvestigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells -
Research • GeneticsHighly Efficient Gene Disruption of Murine and Human Hematopoietic Progenitor Cells by CRISPR/Cas9 -
Research • Cancer ResearchProstate Organoid Cultures as Tools to Translate Genotypes and Mutational Profiles to Pharmacological Responses
CRISPR in HIV
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Research • Immunology and InfectionCRISPR-Cas9-based Genome Engineering to Generate Jurkat Reporter Models for HIV-1 Infection with Selected Proviral Integration Sites -
Research • GeneticsA Protocol for the Production of Integrase-deficient Lentiviral Vectors for CRISPR/Cas9-mediated Gene Knockout in Dividing Cells -
Research • GeneticsAmplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing -
Research • Immunology and InfectionEvaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy -
Research • GeneticsUsing a Fluorescent PCR-capillary Gel Electrophoresis Technique to Genotype CRISPR/Cas9-mediated Knockout Mutants in a High-throughput Format
CRISPR in tweaking the disease genome
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Research • GeneticsIntroducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System -
Research • GeneticsOverexpressing Long Noncoding RNAs Using Gene-activating CRISPR -
Research • GeneticsEmbryo Microinjection Techniques for Efficient Site-Specific Mutagenesis in Culex quinquefasciatus -
Research • Immunology and InfectionLentiviral CRISPR/Cas9-Mediated Genome Editing for the Study of Hematopoietic Cells in Disease Models -
Research • Immunology and InfectionGenerating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
CRISPR in helping IVF and emryo transfer technology
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Research • GeneticsUse of Freeze-thawed Embryos for High-efficiency Production of Genetically Modified Mice -
Research • BioengineeringProduction of Genetically Engineered Golden Syrian Hamsters by Pronuclear Injection of the CRISPR/Cas9 Complex -
Research • BiologySilencing the Spark: CRISPR/Cas9 Genome Editing in Weakly Electric Fish -
Research • GeneticsCRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
CRISPR in improving crop yield and plant biotechnology
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Research • BiologyAgrobacterium-Mediated Genetic Transformation, Transgenic Production, and Its Application for the Study of Male Reproductive Development in Rice -
Research • BiologyHigh-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots -
Research • GeneticsTransforming, Genome Editing and Phenotyping the Nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii -
Research • GeneticsA Robotic Platform for High-throughput Protoplast Isolation and Transformation -
Research • BioengineeringMicroinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
CRISPR in stem cell applications/transgenic models
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Research • GeneticsEndogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9 -
Research • Developmental BiologyEstablishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation -
Research • Developmental BiologyGenerating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells -
Research • Developmental BiologyCRISPR/Cas9 Technology in Restoring Dystrophin Expression in iPSC-Derived Muscle Progenitors -
Research • GeneticsIntroducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing
