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JoVE Journal
Genetics

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转化、基因组编辑和拟合氮的热带大麻树帕拉斯波尼亚安德森
 
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转化、基因组编辑和拟合氮的热带大麻树帕拉斯波尼亚安德森

Article DOI: 10.3791/59971
August 18th, 2019

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Summary August 18th, 2019

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寄生虫树是一种快速生长的热带树,属于大麻家族(大麻),可以形成固氮根结节与rhizobium。在这里,我们描述了基于农业细菌-肿瘤介导的稳定转化和CRISPR/Cas9基于基因组编辑的P.andersonii中反向遗传分析的详细方案。

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