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Journal / Genetics / Trasformazione, editing del genoma e…
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Genetics

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JoVE Journal Genetics
Trasformazione, editing del genoma e fenotipizzazione dell'azoto-fixing Tropical Cannabaceae Tree Parasponia andersonii
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Trasformazione, editing del genoma e fenotipizzazione dell'azoto-fixing Tropical Cannabaceae Tree Parasponia andersonii

Article DOI: 10.3791/59971-v • 12:22 min • August 18th, 2019
August 18th, 2019 •
1,2, 1,2, 1,3, 1,4, 1, 1, 1, 1
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 2Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

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Summary
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Parasponia andersonii è un albero tropicale in rapida crescita che appartiene alla famiglia Cannabis (Cannabaceae) e può formare noduli di radice che fissano l'azoto in associazione con il rizobium. Qui, descriviamo un protocollo dettagliato per le analisi genetiche inverse in P. andersonii basato su Agrobacterium tumefaciens- mediato trasformazione stabile e modifica del genoma CRISPR/Cas9.

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Genetica Numero 150 Parasponia andersonii Cannabaceae albero legno Agrobacterium tumefaciens trasformazione stabile CRISPR-Cas9 editing del genoma nodulation rizobium micorrino
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