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Immunology and Infection

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JoVE Journal Immunology and Infection
Lentiviral CRISPR/Cas9-Vermittelte Genombearbeitung zur Untersuchung hämatopoetischer Zellen in Krankheitsmodellen
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Lentiviral CRISPR/Cas9-Vermittelte Genombearbeitung zur Untersuchung hämatopoetischer Zellen in Krankheitsmodellen

Article DOI: 10.3791/59977-v • 08:14 min • October 3rd, 2019
October 3rd, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

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Beschrieben werden Protokolle für die hocheffiziente Genombearbeitung von murinen hämatopoetischen Stamm- und Vorläuferzellen (HSPC) durch das CRISPR/Cas9-System zur schnellen Entwicklung von Mausmodellsystemen mit hämatopoetischen systemspezifischen Genmodifikationen.

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Immunologie und Infektion Ausgabe 152 CRISPR/Cas9 Cas9 transgene Maus Genombearbeitung murine hämatopoetische Stammzelle Lentivirus Knochenmarktransplantation
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