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Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique
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Murine Cervical Aortic Transplantation Model using a Modified Non-Suture Cuff Technique

Article DOI: 10.3791/59983-v • 10:11 min • November 2nd, 2019
November 2nd, 2019 •
1, 1, 1, 1, 2, 1, 1, 1, 1, 1, 1, 1, 1, 1,3
1Department of General, Visceral, and Transplant Surgery, Ludwig Maximilian University of Munich, 2Walter-Brendel-Centre of Experimental Medicine, Ludwig Maximilian University of Munich, 3Department of General, Visceral and Cancer Surgery, University Hospital of Cologne, University of Cologne

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Summary
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Here, we present a protocol of heterotopic aortic transplantation in mice using the non-suture cuff technique in a cervical murine model. This model can be used to study the underlying pathology of chronic allograft vasculopathy (CAV) and can help evaluate new therapeutic agents in order to prevent its formation.

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Murine Cervical Aortic Transplantation Modified Non-suture Cuff Technique Research Laboratories Department Of General Visceral And Transplant Surgery LMU Munich Heterotopic Aortic Transplantation Model Anesthetize Intraperitoneal Injection Midazolam Medetomidine Fentanyl Electric Hair Clipper Ophthalmic Ointment Heating Pad Microscope Operating Table Skin Incision Jugular Incision Right Lower Mandible Bipolar Cautery Vessel Pedicle Micro Scissors Sternocleidomastoid Muscle Common Carotid Artery
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