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Journal / Biochemistry / En tilpasningsvennlig tilnærming for enzymatisk…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
En tilpasningsvennlig tilnærming for enzymatisk produksjon og rensing av Diterpenoid naturlige produkter
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En tilpasningsvennlig tilnærming for enzymatisk produksjon og rensing av Diterpenoid naturlige produkter

Article DOI: 10.3791/59992-v • 07:59 min • October 4th, 2019
October 4th, 2019 •
1, 1, 1, 1, 1, 1
1Department of Plant Biology, University of California Davis

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Her presenterer vi lett å bruke protokoller for å produsere og rense diterpenoid metabolitter gjennom Kombinatorisk uttrykk for biosyntetiske enzymer i Escherichia coli eller Nicotiana benthamiana, etterfulgt av kromatografiske produkt Rensing. De resulterende metabolitter er egnet for ulike studier inkludert molekylær struktur karakterisering, enzym funksjonelle studier, og bioactivity analyser.

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Biokjemi diterpenoid biosyntesen co-uttrykk diterpene syntase cytokrom P450 monooxygenase Nicotiana benthamiana Zea Mays plante spesialisert metabolisme
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