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Journal / Neuroscience / 使用CRISPR-Cas9在蜜蜂脑科进行抗RDL和抗mGGR1受体抗体测试…
JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
使用CRISPR-Cas9在蜜蜂脑科进行抗RDL和抗mGGR1受体抗体测试
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使用CRISPR-Cas9在蜜蜂脑科进行抗RDL和抗mGGR1受体抗体测试

Article DOI: 10.3791/59993-v • 09:25 min • January 30th, 2020
January 30th, 2020 •
1,2, *2, *2, 3, 2, 1, 4, 4, 2
1Department of Neuroscience, University of Arizona, 2School of Life Sciences, Arizona State University, 3Department of Scientific and Technologic Investigations, University of Sonora, 4Integrated DNA Technologies, Inc.
* These authors contributed equally

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Summary
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这里介绍的是一个协议，使用CRISPR-Cas9系统减少成人蜜蜂大脑中蛋白质的产生，以测试抗体特异性。

Tags

神经科学， 问题 155， GABAA受体， RDL， 代谢性谷氨酸受体， mGlutR1， 结合抗体对照， CRISPR-Cas9
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