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Specifieke etikettering van Mitochondriale nucleoïden voor time-lapse Structured Illumination Microscopie
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Specifieke etikettering van Mitochondriale nucleoïden voor time-lapse Structured Illumination Microscopie

Article DOI: 10.3791/60003-v • 07:53 min • June 4th, 2020
June 4th, 2020 •
1, 1, 1
1Imaging Facility, Max Planck Institute of Immunobiology and Epigenetics

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Het protocol beschrijft specifieke etikettering van mitochondriale nucleoïden met een commercieel verkrijgbare DNA-gel vlek, verwerving van time lapse-serie van live gelabelde cellen door super-resolutie gestructureerde verlichting microscopie (SR-SIM), en automatische tracking van nucleoïde beweging.

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Biologie mitochondriale nucleoïde mitochondriaal DNA asymmetrische cyanine DNA fluorescerende vlek gestructureerde verlichtingsmicroscopie levende celmicroscopie
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