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Specifik mærkning af mitokondrielle nukleoider til tidsforfald struktureret belysning mikroskopi
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Specifik mærkning af mitokondrielle nukleoider til tidsforfald struktureret belysning mikroskopi

Article DOI: 10.3791/60003-v • 07:53 min • June 4th, 2020
June 4th, 2020 •
1, 1, 1
1Imaging Facility, Max Planck Institute of Immunobiology and Epigenetics

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Protokollen beskriver specifik mærkning af mitokondrielle nukleoider med en kommercielt tilgængelig DNA gel plet, erhvervelse af tid bortfalder serie af levende mærket celler ved super-opløsning struktureret belysning mikroskopi (SR-SIM), og automatisk sporing af nukleoid bevægelse.

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Biologi mitokondriel nukleoid mitokondrielt DNA asymmetrisk cyanin DNA fluorescerende plet struktureret belysning mikroskopi levende celle mikroskopi
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