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JoVE Journal Biochemistry
En semi-High-genomströmning anpassning av NADH-kopplad ATPas-analys för screening av småmolekylära hämmare
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En semi-High-genomströmning anpassning av NADH-kopplad ATPas-analys för screening av småmolekylära hämmare

Article DOI: 10.3791/60017-v • 10:28 min • August 17th, 2019
August 17th, 2019 •
1,2, 1,2, 3, 2, 1,2
1Department of Molecular Medicine, The Scripps Research Institute, 2Department of Neuroscience, The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI, National Institutes of Health

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En nikotinamid-adenin-dinukleotid (NADH)-kopplad ATPas-analys har anpassats till semihigh genomströmning screening av små molekyler myosin-hämmare. Denna kinetiska analys körs i ett 384-väl mikroplattformat med totala reaktions volymer på endast 20 μL per brunn. Plattformen bör vara tillämplig på praktiskt taget alla ADP-producerande enzym.

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Biokemi ATPas assay NADH fluorescens semi High-genomströmning screening hämmande konstant myosin
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