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Journal / Genetics / 使用基于循环 RT-PCR 的策略对玉米 Mitochondrion…
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Genetics

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JoVE Journal Genetics
使用基于循环 RT-PCR 的策略对玉米 Mitochondrion 中初级和已处理转录的区分和映射
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Journal (Genetics)
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使用基于循环 RT-PCR 的策略对玉米 Mitochondrion 中初级和已处理转录的区分和映射

Article DOI: 10.3791/60019-v • 07:26 min • July 29th, 2019
July 29th, 2019 •
1,2, 2, 2, 2, 2, 1,2, 1,2, 1,2
1State Key Laboratory of Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, 2Guangdong Provincial Key Laboratory of Plant Molecular Breeding, College of Agriculture, South China Agricultural University

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通过结合循环RT-PCR、定量RT-PCR、RNA 5'聚磷酸酶处理和北方斑点,提出了基于RT-PCR的循环RT-PCR策略。该协议包括一个标准化步骤,以尽量减少不稳定5'三磷酸酯的影响,它适用于对玉米线粒体中稳定积累的初级和加工转录转录进行鉴别和映射。

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遗传学 问题 149 圆形 RT-PCR 北方斑点 定量RT-PCR RNA 5' 聚磷酸酶处理 RNA正常化 初级和加工转录本 玉米线粒体
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