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JoVE Journal Cancer Research
Het verkrijgen van kanker stamcelbollen van gynaecologische en borstkankertumoren
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Het verkrijgen van kanker stamcelbollen van gynaecologische en borstkankertumoren

Article DOI: 10.3791/60022-v • 07:01 min • March 1st, 2020
March 1st, 2020 •
*1,2,3,4, *1,2,3,4,5,6, 1,2,3, 2,7, 1,2,3,4,8, 9, 3,9,10, 1,2,3,4,8
1Institute of Biophysics, Faculty of Medicine, University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, 6Gynecology A Service, Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences
* These authors contributed equally

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Het doel van deze methodologie is om kankercellen (CSC) te identificeren in kankercellijnen en primaire menselijke tumormonsters met het bolvormende protocol, op een robuuste manier, met behulp van functionele testen en fenotypische karakterisering met stroomcytometrie en westerse Vlek.

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Cancer Research neoplastische stamcellen borstneoplasma's tumorsferen gynaecologische kanker sfeervormingsprotocol kankerstamcelmarkers
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