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JoVE Journal Biochemistry
Kwantificering van eiwit interactie netwerk dynamiek met Multiplexed co-immunoprecipitatie
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Kwantificering van eiwit interactie netwerk dynamiek met Multiplexed co-immunoprecipitatie

Article DOI: 10.3791/60029-v • 07:57 min • August 21st, 2019
August 21st, 2019 •
1,2, 3,4, 5, 6,7,8, 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

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Kwantitatieve multiplex Immunoprecipitation (QMI) gebruikt Flowcytometrie voor gevoelige detectie van verschillen in de overvloed van gerichte eiwit-eiwit interacties tussen twee monsters. QMI kan worden uitgevoerd met een kleine hoeveelheid biomateriaal, vereist geen genetisch gemanipuleerde Tags en kan worden aangepast voor elk eerder gedefinieerd proteïne-interactie netwerk.

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Biochemie uitgave 150 IP-FCM proteomics eiwit interactie signalering Immunoprecipitation QMI
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