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Journal / Biochemistry / 멀티플렉스 동시 면역 침전을 이용한 단백질 상호 작용 네트워크 역학의…
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Biochemistry

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JoVE Journal Biochemistry
멀티플렉스 동시 면역 침전을 이용한 단백질 상호 작용 네트워크 역학의 정량화
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Journal (Biochemistry)
A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation
Laboratory Scale Production and Purification of a Therapeutic Antibody
Journal (Biochemistry)
Laboratory Scale Production and Purification of a Therapeutic Antibody
 
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멀티플렉스 동시 면역 침전을 이용한 단백질 상호 작용 네트워크 역학의 정량화

Article DOI: 10.3791/60029-v • 07:57 min • August 21st, 2019
August 21st, 2019 •
1,2, 3,4, 5, 6,7,8, 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

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정량적 멀티플렉스 면역침전(QMI)은 두 샘플 간의 표적 단백질-단백질 상호 작용의 풍부성에 대한 민감한 검출을 위해 유세포분석기를 사용합니다. QMI는 소량의 생체 물질을 사용하여 수행될 수 있고, 유전자 조작 태그를 필요로 하지 않으며, 이전에 정의된 단백질 상호작용 네트워크에 적응될 수 있다.

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생화학 문제 150 IP-FCM 단백질 대사 신호 면역 침전 QMI
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