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Journal / Biochemistry / Quantificação da dinâmica da rede de interação…
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JoVE Journal Biochemistry
Quantificação da dinâmica da rede de interação protéica utilizando co-imunoprecipitação multiplexada
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Quantificação da dinâmica da rede de interação protéica utilizando co-imunoprecipitação multiplexada

Article DOI: 10.3791/60029-v • 07:57 min • August 21st, 2019
August 21st, 2019 •
1,2, 3,4, 5, 6,7,8, 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

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A imunoprecipitação quantitativa Multiplex (QMI) utiliza citometria de fluxo para detecção sensível de diferenças na abundância de interações proteína-proteína direcionadas entre duas amostras. O QMI pode ser realizado usando uma pequena quantidade de biomaterial, não requer Tags geneticamente projetadas e pode ser adaptado para qualquer rede de interação protéica previamente definida.

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Bioquímica edição 150 IP-FCM proteômica interação proteica sinalização imunoprecipitação QMI
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