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Journal / Biochemistry / Multipleksed Co-İmmünopreyağış Kullanarak Protein …
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Biochemistry

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JoVE Journal Biochemistry
Multipleksed Co-İmmünopreyağış Kullanarak Protein Etkileşim Ağı Dinamiğinin Ölçülmesi
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Journal (Biochemistry)
Automated Acoustic Dispensing for the Serial Dilution of Peptide Agonists in Potency Determination Assays
Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems
Journal (Biochemistry)
Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems
 
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Multipleksed Co-İmmünopreyağış Kullanarak Protein Etkileşim Ağı Dinamiğinin Ölçülmesi

Article DOI: 10.3791/60029-v • 07:57 min • August 21st, 2019
August 21st, 2019 •
1,2, 3,4, 5, 6,7,8, 1,2,9
1Center for Integrative Brain Research, Seattle Children's Research Institute, 2Graduate Program in Neuroscience, University of Washington, 3Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute, Department of Medicine, Harvard Medical School, 4Broad Institute of Harvard and MIT, 5Department of Mathematics, University of Houston, 6Department of Molecular Microbiology and Immunology, School of Medicine, University of Missouri, 7Department of Surgery, School of Medicine, University of Missouri, 8Department Bioengineering, College of Engineering, University of Missouri, 9Department of Pediatrics, University of Washington

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Kantitatif Multipleks İmmünopantü (QMI), iki örnek arasındaki hedeflenen protein-protein etkileşimlerinin bolluğundaki farklılıkların hassas tespiti için akış sitometrisini kullanır. QMI biyomalzeme küçük bir miktar kullanılarak yapılabilir, genetiği değiştirilmiş etiketleri gerektirmez, ve daha önce tanımlanmış herhangi bir protein etkileşim ağı için adapte edilebilir.

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Biyokimya Sayı 150 IP-FCM proteomik protein etkileşimi sinyalizasyon immünoprepitasyon QMI
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