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JoVE Journal
Genetics

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单分子Fürster共振能量传递方法,用于GEN1对霍利迪结分辨率进行实时调查
 
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单分子Fürster共振能量传递方法,用于GEN1对霍利迪结分辨率进行实时调查

Article DOI: 10.3791/60045
September 18th, 2019

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Summary September 18th, 2019

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Click here for the English version.

这里介绍的是一个执行单分子Fürster共振能量转移以研究HJ分辨率的协议。双色交替激励用于确定解离常数。然后,在实时裂解测定中应用单色时移smFRET,以获得HJ分辨率前的沉思时间分布。

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