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Immunology and Infection

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JoVE Journal Immunology and Infection
Inducing meningokokk hjernehinnebetennelse serogruppe C i mus via Intracisternal Delivery
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Inducing meningokokk hjernehinnebetennelse serogruppe C i mus via Intracisternal Delivery

Article DOI: 10.3791/60047-v • 10:03 min • November 5th, 2019
November 5th, 2019 •
1, 1, 1, 1, 1, 1, 2, 1, 1,3
1Department of Molecular Medicine and Medical Biotechnology, University of Naples Federico II, 2Department of Science and Technology, Sannio University, 3CEINGE – Advanced Biotechnology

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Her beskriver vi en metode for å indusere meningokokk meningitt gjennom en intracisternal ruten av infeksjon i voksen mus. Vi presenterer en trinnvis protokoll for meningokokkinfeksjon fra utarbeidelse av inokulum til intracisternal infeksjon; deretter registrere dyret overlevelse og evaluere bakteriell belastninger i murine vev.

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Immunologi og infeksjon infeksjon Neisseria meningitidis meningokokk hjernehinnebetennelse mus modeller intra-cisternal injeksjon hjernevev isogenic mutant belastning.
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