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Immunology and Infection

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JoVE Journal Immunology and Infection
Adoptivimmunterapi av iNKT-celler i glucose-6-fosfat isomerase (G6PI)-induserte RA-mus
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Adoptivimmunterapi av iNKT-celler i glucose-6-fosfat isomerase (G6PI)-induserte RA-mus

Article DOI: 10.3791/60048-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1,2, *1,2, *1,2, *1,2, *1,2, *1,2, *1,2, *1,2, *1,2
1Medical School of Hebei University, 2Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases in Hebei Province
* These authors contributed equally

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Denne protokollen bruker G6PI blandede peptider for å konstruere revmatoid artritt modeller som er nærmere den av menneskelig revmatoid artritt i CD4+ T celler og cytokiner. Høy renhet invariant naturlige killer T celler (hovedsakelig iNKT2) med spesifikke fenotyper og funksjoner ble oppnådd ved in vivo induksjon og in vitro rensing for adoptivimmunterapi.

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Immunologi og infeksjon Utgave 155 revmatoid artritt adoptivimmunterapi iNKT-celler cytokiner glukose-6-fosfat isomerase (G6PI) in vivo bildesystem (IVIS)
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