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JoVE Journal
Genetics

This content is Open Access.

使用蝇S2细胞进行细胞分裂的活成像
 
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使用蝇S2细胞进行细胞分裂的活成像

Article DOI: 10.3791/60049-v 06:17 min August 23rd, 2019
August 23rd, 2019

Chapters

Summary

Please note that all translations are automatically generated.

Click here for the English version.

使用荧光标记的蛋白质和延时显微镜可以实时可视化细胞分裂。使用此处介绍的协议,用户可以分析细胞分裂时序动力学、线粒主轴组件以及染色体的组装和分离。RNA干扰(RNAi)介导的基因敲除后这些事件的缺陷可以进行评估和量化。

Tags

遗传学, 问题 150,果蝇,S2 细胞, 米毒, 细胞周期, 活细胞成像, 线心主轴, 染色体动力学, mCherry: β-图布林, GFP:CENP-A, CID
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