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JoVE Journal
Genetics

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使用蝇S2细胞进行细胞分裂的活成像
 
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使用蝇S2细胞进行细胞分裂的活成像

Article doi: 10.3791/60049
August 23rd, 2019

Summary August 23rd, 2019

Please note that all translations are automatically generated.

Click here for the English version.

使用荧光标记的蛋白质和延时显微镜可以实时可视化细胞分裂。使用此处介绍的协议,用户可以分析细胞分裂时序动力学、线粒主轴组件以及染色体的组装和分离。RNA干扰(RNAi)介导的基因敲除后这些事件的缺陷可以进行评估和量化。

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