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Journal / Developmental Biology / Disseksjon, kultur og analyse av primær skallen…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Disseksjon, kultur og analyse av primær skallen neural Crest celler fra mus for studiet av Neural Crest Cell delaminering og migrasjon
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Disseksjon, kultur og analyse av primær skallen neural Crest celler fra mus for studiet av Neural Crest Cell delaminering og migrasjon

Article DOI: 10.3791/60051-v • 09:33 min • October 3rd, 2019
October 3rd, 2019 •
*1,2, *1,3, 1, 1, 1,3, 2,4, 3, 1
1Centre for Craniofacial and Regenerative Biology, King's College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics, King's College London, 4Department of Biological Applications and Technology, University of Ioannina
* These authors contributed equally

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Denne protokollen beskriver disseksjon og kulturen i skallen nevrale kam celler fra musemodeller, primært for studiet av celle migrasjon. Vi beskriver Live Imaging teknikker som brukes og analyse av hastighet og celle formendringer.

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Utviklingsbiologi cellekultur Live Imaging mus skallen neural Crest celle migrasjon epitel-mesenchymal overgang celle motilitet
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