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JoVE Journal
Genetics

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Forbedring af små RNA-SEQ
 
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Forbedring af små RNA-SEQ: mindre bias og bedre påvisning af 2 '-O-methyl-RNAs

Article DOI: 10.3791/60056-v 08:49 min September 16th, 2019
September 16th, 2019

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

Vi præsenterer en detaljeret lille RNA bibliotek reparation protokol med mindre bias end standardmetoder og en øget følsomhed for 2 '-O-methyl RNAs. Denne protokol kan følges ved hjælp af hjemmelavede reagenser for at spare omkostninger eller bruge kits til bekvemmelighed.

Tags

Genetik lille RNA lille RNA-SEQ bias bibliotek forberedelse næste generations sekventering NGS 2 '-O-methyl (2 '-OMe) RNA plante microRNA Plant miRNA
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