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Journal / Genetics / 改进小RNA-seq：减少偏差，更好地检测2'-O-甲基RNA…
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Genetics

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JoVE Journal Genetics
改进小RNA-seq：减少偏差，更好地检测2'-O-甲基RNA
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改进小RNA-seq：减少偏差，更好地检测2'-O-甲基RNA

Article DOI: 10.3791/60056-v • 08:49 min • September 16th, 2019
September 16th, 2019 •
1, 1, 1
1Institute for Integrative Biology of the Cell, UMR9198, CNRS CEA Univ Paris-Sud, Université Paris-Saclay

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我们提出了详细的小RNA库修复协议，比标准方法的偏差小，对2'-O-甲基RNA的灵敏度提高。使用自制试剂可节省成本或使用试剂盒以方便使用本协议。

Tags

遗传学， 问题 151， 小RNA， 小RNA-seq， 偏置， 库制备， 下一代测序， NGS， 2'-O-甲基（2'-OMe） RNA， 植物微RNA， 植物miRNA
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