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Journal / Immunology and Infection / Ex Vivo Transmigration Sisteminde Lenfosit…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Ex Vivo Transmigration Sisteminde Lenfosit Göçünün Değerlendirilmesi
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Ex Vivo Transmigration Sisteminde Lenfosit Göçünün Değerlendirilmesi

Article DOI: 10.3791/60060-v • 10:25 min • September 20th, 2019
September 20th, 2019 •
1,3, 2,3,4
1Department of Internal Medicine, University of Utah, 2Research Service, VA Nebraska Iowa Health Care System, 3Department of Internal Medicine, University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health, University of Nebraska Medical Center

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Bu protokolde lenfositler, alt odadan gözenekli bir zarla ayrılan bir transmigration sisteminin üst odasına yerleştirilir. Keokin alt hazneye eklenir, bu da bir kemokin gradyan boyunca aktif göçe neden olur. 48 saat sonra, lenfositler transmigration quantitate için her iki odada sayılır.

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İmmünoloji ve Enfeksiyon Sayı 151 CCL17 (C-C Motif Kemokin 17)/TARC (timus ve aktivasyona bağlı kemokin) CCL22 (C-C Motif Kemokin 22)/MDC (Makrofaj kaynaklı kemokin) CCR4 (CCCc Kemokin Reseptör 4) CD4 T hücresi (CD4+ Thelper hücre)/Th2 hücre ILC2: (Grup 2 Doğuştan Lenfoid Hücre) OVA (Tavuk Yumurtaovalbumin)
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