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JoVE Journal
Immunology and Infection

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Vurdering af lymfocyt migration i et ex vivo-Transmigrerings system
 
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Vurdering af lymfocyt migration i et ex vivo-Transmigrerings system

Article DOI: 10.3791/60060-v
September 20th, 2019

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Summary September 20th, 2019

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I denne protokol er lymfocytter placeret i det øverste kammer af et transmigrerings system, adskilt fra det nederste kammer af en porøs membran. Chemokin tilsættes til det nederste kammer, som inducerer aktiv migration langs en chemokine gradient. Efter 48 h tælles lymfocytter i begge kamre for at kvantificere overførsel.

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