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JoVE Journal
Immunology and Infection

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外迁移系统中淋巴细胞迁移的评估
 
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外迁移系统中淋巴细胞迁移的评估

Article DOI: 10.3791/60060-v
September 20th, 2019

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Summary September 20th, 2019

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在此协议中,淋巴细胞被放置在迁移系统的顶室中,由多孔膜与底部腔室隔开。化学因子被添加到底部腔室,从而诱导沿化学基质梯度的活性迁移。48小时后,淋巴细胞在两个腔室计数,以定量迁移。

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