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Journal / Immunology and Infection / Bewertung der Lymphozytenmigration in einem…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Bewertung der Lymphozytenmigration in einem Ex-Vivo-Transmigrationssystem
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Bewertung der Lymphozytenmigration in einem Ex-Vivo-Transmigrationssystem

Article DOI: 10.3791/60060-v • 10:25 min • September 20th, 2019
September 20th, 2019 •
1,3, 2,3,4
1Department of Internal Medicine, University of Utah, 2Research Service, VA Nebraska Iowa Health Care System, 3Department of Internal Medicine, University of Nebraska Medical Center, 4Department of Environmental, Agricultural & Occupational Health, University of Nebraska Medical Center

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In diesem Protokoll werden Lymphozyten in die obere Kammer eines Transmigrationssystems gelegt, die durch eine poröse Membran von der unteren Kammer getrennt sind. Chemokin wird der unteren Kammer hinzugefügt, was eine aktive Migration entlang eines Chemokingradienten induziert. Nach 48 h werden Lymphozyten in beiden Kammern gezählt, um die Transmigration zu quantitieren.

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Immunologie und Infektion Ausgabe 151 CCL17 (C-C Motiv Chemokin 17)/TARC (Thymus und Aktivierungsbezogenes Chemokin) CCL22 (C-C Motiv Chemokin 22)/MDC (Macrophage-derived chemokine) CCR4 (CC Chemokine Receptor 4) CD4 T Cell (CD4+ T helper cell)/Th2 cell ILC2: (Gruppe 2 angeborene Lymphoidzelle) OVA (Chicken Egg Ovalbumin)
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