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Journal / Immunology and Infection / Purification de l'homme S100A12 et de ses…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification de l'homme S100A12 et de ses oligomères induits par les ions pour la stimulation cellulaire immunitaire
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Purification de l'homme S100A12 et de ses oligomères induits par les ions pour la stimulation cellulaire immunitaire

Article DOI: 10.3791/60065-v • 12:55 min • September 29th, 2019
September 29th, 2019 •
1, 1, 1
1Department of Pediatric Rheumatology and Immunology, University Children's Hospital

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Ce protocole décrit une méthode de purification pour la protéine recombinante sans étiquette de liaison de calcium S100A12 et ses oligomères ion-induits pour des essais humains de stimulation de monocyte.

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Immunologie et infection numéro 151 S100A12 DAMP purification des protéines chromatographie chromatographie d'échange d'anion chromatographie hydrophobe-interaction chromatographie de taille-exclusion liaison syrochimique stimulation monocyte
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