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Génération de modifications génomiques définies à l'aide de CRISPR-CAS9 dans les cellules souches pluripotentes humaines
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Génération de modifications génomiques définies à l'aide de CRISPR-CAS9 dans les cellules souches pluripotentes humaines

Article DOI: 10.3791/60085-v • 09:04 min • September 25th, 2019
September 25th, 2019 •
*1, *1, 1,2,3, 1,2,3
1Center for Cellular and Molecular Therapeutics, The Children's Hospital of Philadelphia, 2Department of Pathology and Laboratory Medicine, The Children's Hospital of Philadelphia, 3Department of Pathology and Laboratory Medicine, University of Pennsylvania
* These authors contributed equally

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Ce protocole fournit une méthode pour faciliter la génération de changements définis de nucléotide hétérozygote ou homozygote à l'aide de CRISPR-CAS9 dans les cellules souches pluripotentes humaines.

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Génétique Numéro 151 cellules souches édition du génome CRISPR-CAS9 oligonucléotide à brin unique mutation hétérozygote lignée cellulaire isogénique
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