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Journal / Neuroscience / Adsorción molecular inducida por la luz de…
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Neuroscience

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JoVE Journal Neuroscience
Adsorción molecular inducida por la luz de proteínas utilizando el sistema PRIMO para micro-patrones para estudiar las respuestas celulares a las proteínas de matriz extracelular
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Adsorción molecular inducida por la luz de proteínas utilizando el sistema PRIMO para micro-patrones para estudiar las respuestas celulares a las proteínas de matriz extracelular

Article DOI: 10.3791/60092-v • 09:49 min • October 11th, 2019
October 11th, 2019 •
*1, *1, 1, 2, 3,4, 5, 1
1Faculty of Biology, Medicine and Health. Division of Cell Matrix, Biology and Regenerative Medicine, Wellcome Trust Centre for Cell-Matrix Research, The University of Manchester, 2School of Materials, The University of Manchester, 3Blond McIndoe Laboratories, School of Biological Sciences, Faculty of Biology, Medicine and Health, Division of Cell Matrix Biology and Regenerative Medicine, The University of Manchester, Manchester Academic Health Science Centre, 4Department of Plastic Surgery & Burns, Wythenshawe Hospital, Manchester University NHS Foundation Trust, Manchester Academic Health Science Centre, 5Department of Pathology, UMC Utrecht
* These authors contributed equally

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Nuestro objetivo general es entender cómo las células detectan señales extracelulares que conducen al crecimiento axonal dirigido. Aquí, describimos la metodología de la Adsorción Molecular Inducida por la Luz de Proteínas, utilizada para producir micropatrones definidos de componentes de matriz extracelular con el fin de estudiar eventos específicos que rigen el crecimiento de axón y la búsqueda de rutas.

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Inmunología e infección Número 152 micropatrón patrón micropatrones LIMAP matriz extracelular (ECM) neurona comportamiento celular migración crecimiento axonal búsqueda de trayectorias toma de decisiones
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