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JoVE Journal Medicine
A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice
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A High-Throughput In Situ Method for Estimation of Hepatocyte Nuclear Ploidy in Mice

Article DOI: 10.3791/60095-v • 08:44 min • April 19th, 2020
April 19th, 2020 •
1, 2, 1,3, 1,3
1Centro de Investigación Príncipe Felipe (CIPF), 2Department of Physiology, Development and Neuroscience, University of Cambridge, 3Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM)

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Wir präsentieren eine robuste, kostengünstige und flexible Methode zur Messung von Veränderungen der Hepatozytenzahl und der Kernploidie in festen/kryokonservierten Gewebeproben, die keine Durchflusszytometrie erfordern. Unser Ansatz bietet eine leistungsstarke probenweite Signatur der Leberzytologie, ideal für die Verfolgung des Fortschreitens von Leberverletzungen und Krankheiten.

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Medizin Ausgabe 158 Leber Polyploidie Hepatozyten hochgehaltige Bildgebung Hepatologie Hepatozytenkernfaktor 4 alpha (HNF4)
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